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171573

Genetical and morphological studies on Ficus trees

Article

Last updated: 23 Jan 2023

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Subjects

-

Tags

Forestry and environmental technology
Horticulture

Abstract

In this current study, DNA barcoding and fifteen plant species of Ficus genus belongs to family moracea were used. The plant species were collected from the botanical garden of Antoniadis Alexandria government, Egypt. To authenticate the morphological identification and measure the evolutionary rate among them, rbcL and matK genes were used as genes universal DNA barcoding. The obtained results for rbcL gene amplification success were 93% and the matK gene amplification 80%. The DNA sequencing was got for 14 species of rbcL gene and 11 species for matK gene in addition, the matK and rbcL sequences for all samples were checked by GenBank databases and the accession numbers were detected for all the studied species. Finally, the rbcL was suggested as a plant barcode for its discriminatory power at high taxonomic levels than matK barcode.

DOI

10.21608/jalexu.2021.171573

Keywords

DNA barcoding, Ficus genus, rbcL, matK, morphological, Genetic, GenBank

Authors

First Name

Houssam ELDin

Last Name

Elwakil

MiddleName

-

Affiliation

Department of Agricultural Botany, Faculty of Agriculture (Saba Basha) Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Amera

Last Name

Zaitoun

MiddleName

Fathallah

Affiliation

Department of Agricultural Botany, Faculty of Agriculture (Saba Basha) Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Bothaina

Last Name

Weheda

MiddleName

-

Affiliation

Department of Floriculture, Horticulture Research Institute, Agriculture Research Center, Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Asmaa

Last Name

Abushady

MiddleName

Mohamed

Affiliation

Department of Genetics, Faculty of Agriculture (Ain Shams) University, Cairo, Egypt , Biotechnology School, Nile University, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Khalid

MiddleName

Elsaid

Affiliation

Department of Agricultural Botany, Faculty of Agriculture (Saba Basha) Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Naglaa

Last Name

Ali

MiddleName

AboElmaaty

Affiliation

Department of Floriculture, Horticulture Research Institute, Agriculture Research Center, Alexandria, Egypt

Email

-

City

-

Orcid

-

Volume

26

Article Issue

2

Related Issue

24882

Issue Date

2021-06-01

Receive Date

2021-03-30

Publish Date

2021-06-01

Page Start

60

Page End

73

Print ISSN

1110-5585

Online ISSN

2785-9525

Article File

JALEXU_Volume 26_Issue 2_Pages 60-73.pdf

PDF . 769.1kB

Link

https://jalexu.journals.ekb.eg/article_171573.html

Detail API

https://jalexu.journals.ekb.eg/service?article_code=171573

Order

4

Type

Research papers

Type Code

1,789

Publication Type

Journal

Publication Title

Journal of the Advances in Agricultural Researches

Publication Link

https://jalexu.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023