The  experiments  were  carried out during the years of  2013, 2014  and  2015  in the Flowers   and   Ornamental   Plants   Research   Gardens   of   the   Faculty  of  Agriculture,  Alexandria   University,  Egypt. The objective of this research was to study the effect of  treating the seeds of  Catharanthus  roseus with different  gamma rays doses, i.e.0, 5, 10, 15 and 20 kr and  irrigation with  saline water (0,100 and 150 mM) on the morphological characteristics, proline content, alkaloids (vindolen and catharnthine) content and total carbohydrates content in  the leaves, variations, mutations and peroxidase  isozyme. Data on the effect of gamma radiation, salinity treatments and the  interaction  between  them revealed the followings results.
1-   Some variations in the morphological characteristics, such as habit of growth , leaf size,
      form and colour, stem colour and flower structure  and colour.
2- Highly significant increases in the proline content.
3a- Significant increases in the leaf vindolen content.
3b- Significant increases in the leaf catharnthine content.
4- No clear effect on the total carbohydrate content.

5- Twelve mutated plants with variation in branching, flowering and salt tolerance.