Two field experiments were conducted at the Experimental Station Farm of Faculty of Agriculture (Saba- Basha) Alexandria University. Egypt during 2014/2015 and 2015/2016 growing seasons. The objective of this study was to investigation the effect of organic manure and biofertilization on yield, yield components and grain quality of three wheat cultivars to improve wheat productivity and minimizing of pollution. The results could be summarized as follows. Sids 12 cultivar gave higher spike length, number of spikelets/spoke, grain number/spike 1000- grain weight, grain, straw and biological yields (ton/fed) as well as harvest index (%) than Sakha 94 cultivar. Also, Sids 12 cultivar significantly surpassed Sakha 94 cultivar in crude protein (%) and potassium percentage in both seasons. The addition of organic manure at 10 m³/fed resulted in a significant increment in yield components and crude protein (%) percentages, phosphorus and potassium of wheat grains in both seasons. Significant variation were recorded between the tested biofertilization treatments for plant height, spike length, number of spikes/m², 1000- grain weight in both seasons. The effective treatment for plant height, spike length, grain straw and biological yield (ton/fed) as well as harvest index were obtained by Sids 12 cultivar with adding organic manure at 10 m³/fed, in the both seasons. Sids 12 cultivars with all biofertilizers inoculation surpassed Sakha 94 cultivar for plant height, grain, straw and biological yields (ton)/fed, in the both seasons.