The objective of this study is to investigate grades and quality parameters of cotton as affected by boll position. The study was conducted for two consecutive seasons 2015/2016 and 2016/2017. Variety under consideration is Acala (Gossypium hirsutum). The Plant was divided into three zones; upper zone (A), middle zone (B) and lower zone(C). Every zone was picked separately and subjected to grading according to Sudan Classification System. Fiber tests were carried out under standard conditions (R.H 65±2%, temp. 25±2 Cº).  The results showed that, seed cotton yield obtained from A-zone had better grades than B and C-zones. Grade 1 constituted 40% and 58% of the total for the two seasons, respectively. For C-zone, grade 1 equal 2% and 16% of the total for the first and second season respectively. Other quality parameters of seed index, lint index, fibre length, micronaire value, maturity ratio, and fineness were better at C-zone.