Cotton (Gossypium barbadense L.) is one of the strategic farm crops which is widely cultivated and traded across the world. Rhizoctonia solani Kuhn is the most important fungal disease that caused pre- and post-emergence damping-off, sore shin and root rot of cotton seedlings. The present study was carried out at Faculty of Agriculture, Saba Basha, Alexandria University, Egypt during the period of 2016 to 2018. Fifteen isolates were collacted from Alexandria, Gharbia, Beni Suef (Sids-Giza 95), El-Beheria, Dakahlia, Kafr El Sheikh (Qelen) and Monufia (Beer alsaba) governorates. Data showed that the isolate from Kafer El-Shikh was the highest percentage (98%) forward by Monufya (94%) then Damanhor by 82%. While Giza 92 showed lowest values recorded to by 16.66%, Giza 45 and 94 by average was almost 17%. Data indicated that Giza 80 was more tolerant (23.125%) comparing with the susceptibly one Giza 93 (60.648%) and both Giza 87 and 92 showed moderate tolerant to fungal. Turmeric as extract plant showed the lowest fungal growth (3 cm) in Kafer El-shikh and (2 cm) in Giza 45 isolates. That mean this save natural material could be useful to control the R. solani.