The main purpose of this investigation was to evaluate the growth, yield and quality of Gazelle sugar beet cultivar under calcareous soil conditions. Two field experiments were carried out at Mariut Experimental Station, Desert Research Center, Egypt, during two successive winter growing seasons 2015/2016 and 2016/2017 to study the effect of four concentrations of boron, control (without boron), 33, 55 and 77 mg /L boron applied as foliar spraying at two times during growth seasons and assigned in the vertical plots and six treatments of nitrogen and biofertilizer; control (without adding nitrogen or biofertilizer), 40 kg N/fed., 80 kg N/fed., biofertilizer, 40 kg N/fed. + biofertilizer and  80 kg N/fed. + biofertilizer which occupied the horizontal plots. Obtained results revealed that, all studied characters i.e. leaf area/plant, leaf area index, root length, root diameter, root yield, top yield, root/top ratio, harvest index, total soluble solids %, sucrose (%), sugar yield and apparent purity percentages were significantly affected by either boron or nitrogen and biofertilization. Also, all previous studied traits were significantly affected by the interaction between nitrogen with biofertilizer and born concentrations, except root diameter in the first season, root yield, root/top ratio and harvest index in the two seasons, which were not affected significantly due to the interaction between the studied factors.