The present study was conducted to investigate the effects of salinity, gibberellic acid (GA3) and biofertilizers on growth, flowering and oil yield of Matricaria chamomilla, L. var. " German chamomile " plants. The experiment was designed as a split-split plot design in three replicates. The main plot represented different concentrations of saline irrigation water ( tap water , 3 and 6 dSm-1 ) . The sub-plot was gibberellic acid (GA3) as foliar application at four levels 0.0, 100 and 200 mg L-1. The sub sub-plot represented bio-fertilizers [nitrobine + phosphorein 1:1 w/w] at rates of 0.0,  5.0 and 10.0 g/ plant . The best treatment due to these combination was resulted from  irrigation plants with fresh or moderate saline water  at EC 3 dSm-1 combined with spraying with all levels of GA3 combined with biofertilizer application at 5.0 and 10.0 g/ plant on growth and flowering characteristics. Maximum  oil percentage resulted from  irrigation  with saline water and application the highest levels of   GA3 and biofertilizer. Combination of fresh water, 200 mg L-1 GA3 and 10 g nitrobein + 10 g phosporein per pot produced  the maximum essential oil yield /plant . Key words: Medicinal and Aromatic Plants, Chamomile,  Matricaria chamomilla, L., salinity,