Solidago canadensis is a rhizomatous perennial plants, involved in multiple purpose as one of the most important commercial cut flowers, with landscape importance and much more importance as a highly valuable medicine plants. Therefore, an efficient plant tissue culture protocol for golden rod (Solidago canadensis) was developed from the nodal cuttings explants. Nodal cuttings, after being disinfected superficially, sterilized with different concentrations of mercuric chloride (HgCl2). Another procedure for surface sterlization take place, the above-mentioned explants were immersed in different concentrations of sodium hypocholrite solution (NaOCl). For in vitro initiation stage propagation, surface sterilized explants were cultured on Murashige and Skoog (MS) medium augmented with varied concentrations of both applied plant growth regulants 6-benzylamino purine (BA) and in combination with naphthalene acetic acid (NAA). Different parameters including the mean number of each shoots, shoot length (cm), mean number of leaflets, mean number of roots formed and per propagule were studied during the course of all tested stage. Neoformed shoots of initiation stage were divided into single nodes with one axillary bud per node each which were used for all micropropagation satage (i.e. initiation, multiplication and rhizogenesis). For acclimatization the plantlets produced from rooting stage were transplanted ex vitro in small plastic pots contained an autoclaved mixture of the perlite (0,1,2,3 volume) and peatmoss (0,1,2,3 volume); and one constant volume of washed and autoclaved sand. In general, the present study revealed that BA and NAA at (nil) 0.00 or 0.25 mg/l and 0.00 and 0.50 mg/l, respectively achieved the best results for initiation stage. Meanwhile, fortified medium with BA and NAA at 0.50 and 0.50 mg/l, consecutively, gave rise to the best results for multiplication stage. Regarding rhizogenesis stage, the best results were recorded when the explants were cultured on MS medium plus IBA and NAA at IBA at 1.50 mg/l and NAA at 2.00 mg/l; which led to the highest mean number of roots formed per propagule., each in turn. Neoformed plantlets were acclimatized ex vitro and in vivo vigorously in mixture of perlite and peatmoss at either (1:1) or (1: 2) and (1: 3), respectively, resulted in the highest mean value (100%) of survival percentage/ plant. and successfully showed true-to-type plants.