Two field experiments were carried out at the Experimental Farm of the Faculty of Agriculture (Saba Basha), Alexandria University, at Abees region, Alexandria. Egypt during the seasons of 2012/2013 and 2013/2014 to study the effect of NPK fertilizers, organic manure levels and biofertilizers on the growth parameters, chemical composition, and majors compounds of the essential oil of Parsley (Petroselinum sativum, L.). The applied experimental design was randomize complete blocks design with three replicates. The main results could be summarized as follows: (1) The fertilization treatments differently affected the mean values of all studied characters, (2) The application with organic manure (2 ton)/fed + 50% NPK+ Cerealine + phoshorein and organic manure (4 ton)/fed + 50% NPK + Cerealine + phosphorein significantly increased plant height, leaf area index, fresh and dry weight at two cuts as well as chlorophyll a & b, chemical composition (N, P and K%) and Vitamin C content during both seasons. (3) However, the application organic manure 2 ton org/fed + 75% NPK + phosphorein gave the highest of major's compounds (apiol, myristien, B- pinene and B- phellandrene %) during 2012/2013 season. This investigation suggests the need for more studies concerning the effect of NPK fertilizer organic manure and biofertilization on Parsley plant under different environments using different types of soil to reach the optimum combination to achive the best yield.