This study was carried out in the tissue culture laboratory , the Faculty of Agriculture, Saba basha, Alexandria University, Egypt during the period from 2012 to 2014 . An efficient and reliable protocol for in vitro propagation of croton (Codiaeum    variegatum Gold Dust.) was optimized. However, nodal explants from field grown of Croton (Codiaeum variegatum Gold Dust. )  were used during in vitro culture study for induction of multiple shoots. Nodal explants were inoculated on various initiation or establishment media with different combinations of NAA and BA and the neoformed shoots were cultured on proliferation (multiplication) media for the development of multiple shoots, and the elongation media to elongate of the neoformed shoot. The subsequent elongated shoots were rooted, and acclimatized ex vitro, successfully. The best medium for shoot initiation was WPM medium supplemented with 1.0 mg/l BA. The favourable medium for multiplication was the tested medium augmented with 2.0 mg/l BA and 0.50 mg/l NAA. In addition, the most effective medium for elongation was the used medium enriched with 1.0 mg/l NAA. Furthermore, th in vitro shoots showed healthy root development when the tested medium was supplemented with combination of 2.0 mg/l IBA and 0.50 mg/l NAA (rooting stage).The combination of sand: peat moss (4:1) was used as substratum for the hardening of the in vitro plantlets, as a potting mix, was the best suited mix for the acclimatization of plantlets.