A multi-residue method for the quantification of 9 organochlorine pesticides, one synthetic
pyrithroid and 7 members of PCBs compounds residues in fish is described. The method
involves the application of a modified pesticide manual procedure followed by GC-ECD (Gas
Chromatography coupled with Electron Capture Detector) analysis. The method is validated
according to the European Union SANCO/12495/2011 guidelines and Pesticide Manual of
Analytical methods. The validation levels were 0.01; 0.04; and 0.2 mg /kg for organochlorines
except fenopropathrin (a synthetic pyrithroid insecticide) which has been validated at the
concentration of 0.02, 0.08 and 0.4 mg /kg and for PCBs at 0.005, 0.02 and 0.1 mg/kg.
Acceptable values were obtained for the following parameters: limit of detection LOD (ranged
between 0.003-0.009 mg/kg for organochlorine pesticides and 0.002 mg/kg for PCB's) and limit
of quantification (LOQ) (0.005 mg/kg for PCB's and in the range of 0.01-0.02 mg/kg for
organochlorine compounds). The recovery percentages ranged between 70 and 120%, and the
measurement uncertainty tests was ±40.0%. The method showed to be linear from the LOQ up < br />to the maximum level; 0.1 mg/kg for PCB's and in the range of 0.2-0.4 mg/kg for organochlorine
compounds. These results demonstrate the applicability of this method in the routine practice
for detecting the residues of such compounds in fatty food.