Lagunaria (Lagunaria patersonii) is considered to be a nearly threatened tree in Egypt where there are only few trees facing a very high risk of extinction owing to the effects of pathogens, pollutants and parasites. Hence, this study was conducted to establish a fast in vitro propagation protocol. Seeds, lateral buds and shoot tips were cultured on MS, NN and N6 media with different concentrations of BAP and NAA and their combinations. Results indicated that the optimum medium for the measured characters was MS free growth regulators and The best rooting   medium was 1/4 MS. RAPD analysis was also applied to detect the genetic polymorphism among Lagonaria plantlet and their original parents.