This study was carried out during three successive seasons of 2013/2014, 2014/2015 and 2015/2016 on parent and first two ratoon crops of three banana cultivars namely ‘Williams Zeaf ', ‘Grand Naine' and ‘Hindi' grown in new orchard situated at Bardees region, El- Baliana city, Sohag Governorate, Egypt. The considered banana plants were raised by tissue culture.maximum Assimilation area (m2) was in ‘Williams Zeaf' (38.24) in second ratoon. The highest values Bunch weight (Kg) was (34.86) in ‘Williams Zeaf' in second ratoon. The highest values of Number of hands/bunch were in ‘Williams Zeaf' (14.00) followed by ‘Grand Naine' (13.20). The highest finger weight (g) was (132.02) in second ratoon followed by (123.20) and (104.60), in ‘Williams Zeaf', ‘Grand Naine' and ‘Hindi' respectively, The highest pulp (%) was (74.17%) followed by (72.46% and 67.63%), in ‘Williams Zeaf', ‘Grand Naine' and ‘Hindi' respectively, The highest total soluble solids (tss) percentage was in ‘Williams Zeaf' (21.50%) followed by ‘Grand Naine' (20.88%), The highest total sugar percentage was in ‘Williams Zeaf' (18.45%) followed by ‘Grand Naine' and ‘Hindi' (16.60 %and 14.86%) respectively. It can be recommended that Williams Zeaf banana cultivar must be planted to obtain the high yield with good fruit quality under this area condition.