The simulated body fluid (SBF) induced epitaxial calcium phosphate
growth on demineralized freeze dried bone allograft (DFDBA) xenogeneic bone
matrices as a function of 60Co-gamma sterilization with single shot technique of
15,25, 30 and 40 KGy. Surface morphology by Scanning electron microscope
(SEM) supplemented by EDAX as well as FT-IR analyses were conducted for
the solids pre-, post-irradiation and post-immersion in SBF up to 72 hours. The
ability of the tissues to form bone was predicted through Ca and iP ions
measurements in SBF as a function of immersing times at each applied gammadose.
The epitaxial growth of octacalcium phosphate (OCP) with rose like
morphology characteristically defined 15 KGy as the most effective gamma
dose on inducing biolayer formation. FT-IR analyses correlated precisely with
the degree of denaturation of protein with 30 KGy and the structure reverted to
a state similar to pre-irradiation with 40 KGy.