The present work deals with the structure study of. Eu0.65Sr0.35Mn1- xFexO3 (x= 0.1, 0.5, and 0.7) by X-ray diffraction. These compounds were prepared from pure 99.9% oxides using standard solid state reaction. The final sintering temperature was 1350°C for 72 h. The X-ray analysis showed that all samples have a single perovskite structure phase, which crystallizes in orthorhombic system with space group Pbnm. An increase in the lattice volume was found upon increasing the iron concentration. The mean bond length (Mn/Fe – O) increases linearly with increasing the iron content. Less distortion in MO6 octahedron is obtained for low iron content (x=0.1).