Objective:  This study was intended to evaluate the micro tensile bond strength (μTBS), micro leakage pattern, and micro morphological analysis of new 10-MDP-based universal adhesive/tooth interface and compare it with other two 10-MDP-based adhesive systems before and after storage. Materials and Methods: Two Universal adhesives (Single Bond Universal Adhesive and Ambar Universal) and one all-in-one adhesive (CLEARFIL S3BOND) were used. A total of 102 permanent human molars were used in this study. The teeth were randomly divided into three groups (n=34) according to the type of used adhesive; Ambar Universal (AU), CLEARFIL S3BOND (CTS), and Single Bond Universal (SBU). Each group was then subdivided into two subgroups (n=17) according to the time of testing.              Results:  It was shown that changing time had no statistically significant effect on µTBS (p > 0.05); however, changing group had a statistically significant effect (p < 0.05). Additionally, the combination of changes in group and time had no statistically significant effect on the µTBS (p > 0.05). Furthermore, it was shown that changing group or time did not significantly affect the micro leakage score. Finally, a slight difference was observed between the studied groups mainly in the resin tags; however, all adhesives showed a loss of resin tags after thermo cycling.Conclusions: The new 10-MDP-based universal adhesive had lower bond strength to dentin than the other tested adhesives. However, the new 10-MDP-based universal adhesive/tooth interface had relatively similar micro leakage patterns and micro morphological features in comparison with the other tested adhesives.