Objective: Assessment of the remineralizing efficacy of Bioactive glass-chitosan and Hydroxy Apatite-Chitosan on artificial white spot lesions. Materials and methods: Sixty extracted human permanent teeth were selected for the study. Specimens were divided into four groups: group 1: fluoride varnish (Voco), group 2: Bioactive Glass-Chitosan varnish (Nano gate company), group 3: Hydroxy Apatite-Chitosan varnish (Nano gate company), group 4: control (treated with remineralizing solution). Artificial carious lesions were induced in all the specimens and treated with respective varnishes twice daily for 7 days. The DIAGNOdent pen (Kavo DIAGNOdent pen 2190) readings were taken, and the surface characteristics of the enamel specimens were evaluated by SEM at baseline, after demineralization and after remineralization. Results: SEM evaluation showed favourable surface changes in all the four study groups after remineralization therapy. Intragroup comparison of DIAGNOdent readings showed a highly significant difference between baseline, post demineralization, and post remineralization values. However, the intergroup comparison was statistically nonsignificant. Conclusion: All test agents were comparable in their remineralization potential.