Objective: Autologous Platelet rich plasma (PRP) is used to accelerate orthodontic tooth movement, but its effect is still controversial. Materials and Methods: Twenty-eight male white Vienna rabbits were divided randomly into two main groups control and PRP. Each group included fourteen rabbits. Tooth movement was measured in seven rabbits from each group after 3 and 14 days respectively. Orthodontic tooth movement was generated by closed coil spring ligated between the mandibular first premolar and mandibular central incisors on both sides. The tooth movement was measured by measuring the space between mandibular first and second premolar using an orthodontic gauge and presented in mm.Results: The mean tooth movement after fourteen days in the PRP group was 3.44 mm while mean tooth movement in the control group was 2.53 mm. There was significant difference between PRP group and control group after fourteen days. Conclusion: The PRP could be an effective method for orthodontic tooth movement acceleration.