Submandibular gland (SMG) presents a great model due to extensive postnatal development and is known to be regulated and affected by hormones and growth factor. In this study low dose of Biochanin a (BCA) and 17beta estradiol (E2) application was observed. The pups were randomly divided into four groups; control, BCA, E2, Dimethyl sulfoxide (DMSO) and euthanized at the 15^th postnatal days (PND). The SMG morphogenesis was altered with the compounds of concern.  The results presented by this study give supplementary data to argue that the effect of a perinatal exposure to Biochanin a on the SSG developmental programming could be linked to sex hormone–dependent disorders. Keywords: Biochanin A (BCA), 17beta estradiol (E2), Dimethyl sulfoxide (DMSO), Submandibular salivary gland (SMG), morphogenesis.