Objectives: The aim of this systematic review was to evaluate the microtensile bond strength (µTBS) of multi-mode adhesives to dentin. Also to conduct a meta-analysis to quantify the differences in mean µTBS in previously published manuscripts for Scotchbond Universal adhesive (3M Espe, st. Paul, MN, USA). Methods:An electronic search was performed using National Library of Medicine (MEDLINE/PubMed), Science Direct and EBSCOhost. Laboratory studies evaluating the µTBS of multi-mode adhesives to dentin by either etch-and-rinse (ER) or self-etch (SE) modes were selected. The following categories were excluded during the assessment process: non-English, published before 2005, clinical trials, animal and review studies. Also the studies used multi-mode adhesives in other purposes were excluded. The reviewed manuscripts were  subjected to meta-analysis to quantify the differences in mean µTBS for Scotchbond Universal adhesive using Comprehensive Meta-Analysis software, version 2 (Biostat, Englewood, Nj, USA), at the 95% confidence interval. Results:Only 10 manuscripts fits the inclusion criteria of this systematic review. There are difference in types of restorative materials, cross-head speed and failure mode among the reviewed manuscripts, as well as differences in beam diameter, number and shape. The meta-analysis outcomes of dentin µTBS showed no statistically significant difference between the ER and SE modes for Scotchbond Universal adhesive (p>0.05). Conclusions:It was found that using Scotchbond Universal adhesive in either ER or SE modes didnot affect µTBS to dentin.