Objective: The aim of this study was to evaluate the levels of platelet derived growth factor-AA and vascular endothelial growth factor in both platelet rich plasma and platelet rich fibrin in type II diabetic chronic periodontitis patients before and after conventional non-surgical periodontal therapy using Luminex x-MAP technology. Subjects and Methods: The present study was carried out on 30 patients who were seeking periodontal treatment and attending Oral medicine and Periodontology Department, Faculty of Dentistry, Mansoura University. Ten healthy subjects were chosen as control subjects. The selected subjects were divided into 4 groups as following; group 1 in which ten patients were selected having chronic periodontitis with type II diabetes mellitus their age ranged from 35-50 years old, group 2in which ten patients were selected having chronic periodontitis without type II diabetes mellitus their age ranged from 35-50 years old, group 3 in which ten patients were selected having type II diabetes mellitus without chronic periodontitis their age ranged from 35-50 years old and finally group 4 in which ten healthy subjects without any systemic diseases and with healthy periodontium were selected with age range also between 35-50 years old. Blood samples were collected from all subjects in our study for the preparation of both PRP and PRF at the baseline in all groups and after 4 weeks of the conventional non-surgical periodontal treatment in group 1 and group 2 subjects for the evaluation of the levels of VEGF and PDGF-AA using Luminex x-map technology. Results: In the present study, the evaluation of the levels of PDGF-AA and VEGF in both PRP and PRF showed higher concentration in the patients with chronic periodontitis and type II diabetes mellitus than that in the chronic periodontitis patients without type II diabetes mellitus, and showed higher concentration in the diabetic patients with healthy gingiva than those in the healthy control group. In addition, these levels declined significantly 4 weeks after conventional non-surgical periodontal treatment with an improvement in the periodontal status as judged by the clinical parameters. Also the levels of PDGF-AA and VEGF were found to be higher in PRF than that in PRP in all groups.