Dr. Ali Ashawish¹. Prof. Omar Khashaba¹, Prof. Sherif  Elnagdy², Dr. Bassant El Mowafey^{1 1-Department of Oral Medicine and Periodontology and Diagnosis and X ray.College of Dintistry.mMansuora University. 2-Department of Oral Pathology,College of Dentistry, Mansoura University.} Abstract: Background, aims:Aim of our study was to evaluate the expression of metalloproteinase-2 (MMP-2) in chronic and aggressive type periodontitis before and after scaling and root planning. (Clinical and immue –histochemical study). And correlate it with clinical parameters in healthy and diseases gingival specimens. Subjects and Method:soft tissue biopsies were taken from three study groups (20 patient diagnosed with chronic- periodontitis, 10 patients with Aggressive- periodontitis (localized or generalized), 10 patients with clinically healthy gingiva). Plaque index, gingival index, pocket depth, clinical attachment level and bleeding on probing index were documented. before and after treatment and biopsies of diseased groups and control group, were processed for H&E stain and immune stain for MMP-2. Results: we reported the expression of MMP-2 in all base line specimens included in our study.(meanºSD: CP group 2.70± .47, AP group 2.80± .42, and control group .89± .33). No significant difference was found between CP and AP group ( P1=0.8), a highly significant differences were reported when each diseased group was compared with control group ( P2 and P3 = <0.001). Pathological specimens, from sites that histologicallyexhibit signs of chronic inflammation and connective tissue remodeling, revealed that the expression of (MMP-2)was mainly confined to fibroblast cells.  Conclusion:A direct correlation is observed between the clinical parameters and expression of MMp-2 secreted in the Chronic and Aggressive periodontitis.