Hala Mohamed A. Sameer¹, Mohamad E. Helal², Fathalla F. Belal³, Mohamed Abdel Rahman Mohamed^{4 1}B .Dent (2007), Dentist, Ministry of Health, AljabalAlgharby, Libya            ²Profof  Oral Biology Department, Faculty of Dentistry , Mansoura university. ³Prof.of  Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy,Mansoura University. ⁴Associate Prof of Oral Biology Department, Faculty of Dentistry, Mansoura University.   Aim of the study:This study was conducted to evaluate the ability of an allograft dentin chips treated by freeze-dried when added to neomycin powders on mandibular bone healing in guinea pigs. Materials and methods:24 male guinea pigs animals were used in the study and divided into 2 groups (I, II,). Each group was divided into subgroups A, B, (2, 4, weeks) respectively post surgically. All groups were subjected to a surgical bone defect created in diastema area. Group I, act as a control where no materials added to the cavity, group II , bone defect was filled with dentin chips mixed with neomycin. Animals were scarified at the specific times, mandibles were dissected, decalcified and processed for Hematoxylin   and eosin stain, immunohistochemical stain using IL-6 antibody. Results:Dentin chips mixed with neomycin groups revealed the most expressive results of IL-6 . Conclusion:Dentin chips mixed with neomycinaimed in the healing of bone defect.