Amal .M. HossinEdawadi^a, Nesreen Nabil Abdelsalam^b, Mohammed I. Mourad^c, Essam  FaroukSoussa^d
aB.D.SFaculty of Dentistry, Alzawia University,
^bAssistant Professor of Oral Biology, Faculty of Dentistry Mansoura University,
^cAssistant Professor of Oral Pathology, Faculty of Dentistry, Mansoura University,
^dProfessor of Oral Biology, Faculty of Dentistry, Mansoura University.

Abstract:
Background and objectives:Drug induced gingival overgrowth is known as an adverse effect with three types of drugs. This study was carried out to evaluate the efficacy of mouthwashes containing krameria tincture compared to those of chlorohexidine gluconateon (CHX) the incidence of cyclosporine( CsA) induced  gingival overgrowth(GO). 
Study design:Sixty adult male albino rats were selected and divided into 4 equal groups. Control group received no treatment.The rats of group II were administrated CsA. Meanwhile, rats of groups III and IV were handled as group II and treated with chlorohexidine(CHX)and mouthwash containing krameria respectively. Routine tissue processing was carried out for staining with keratinocyte growth factor(KGF)and connective-tissue growth factor( CTGF).The results of this study were analyzed statistically.
Results:It was found that control group exhibited mild gingival overgrowth( GO) with knife edge gingival crest while group II yielded moderate gingival overgrowth GO with blunt gingival crest (20%) and sever gingival overgrowth( GO) with bulbous gingival crest. Rats of groups III and IV revealed mild gingivalovergrowth (GO) with knife edge gingival crest.Also,Group II had the highest mean values for  keratinocyte growth factor(KGF) and connective tissue growth factor( CTGF) while control group showed the lowest values. Statistically, there was an overall significant difference between the studied groups as well as between each two groups except between groups III and IV.
Conclusion:From the findings of the present study, we can conclude that chlorohexidine(CHX) and krameria tincture containing mouthwashes might play essential biological role in the reduction of drugs induced gingival overgrowth (DIGO) progression through their effects on the expression of keratinocyte growth factor( KGF) and connective tissue growth factor( CTGF).