Background: The concept of infection-based carcinogenesis is gaining an increasing attention. Also periodontitis has been extensively studied as possible risk factor for gastrointestinal cancers including oral cancer. Methodology: Using conventional PCR, we detected the presence of the key periodontal pathogen Porphyromonas gingivalis (P. gingivalis) in 25 oral cancer fresh tissue specimens versus 25 healthy oral mucosal tissue..   Results: P. gingivalis showed significantly higher prevalence in control group with slightly higher loads in cancer tissue. Bacterial presence was not found to be correlated with tumor progression. Conclusion: P. gingivalis was not found to play a possible role in the cancerous lesion. More studies should be implemented to investigate the bacterial involvement in the oral tumors.