Fatty fish and fish bone have been used successfully in the traditional management of Osteoporosis. The present study was carried out to investigate the role of sibia, sardine and salmon fish as an anti-osteoporotic effect in female rats. Fifty adult female albino rats were divided into five groups as follows:The first group (10 rats) was kept as a negative control group and were fed only on a basal diet. The other four groups (10 rats each) were fed on a basal diet containing 100 mg Prednisone Acetate as a source of glucocorticoid/ kg diet for two weeks to induce osteoporosis, four rats were selected to insure induction of osteoporosis by DEXA scan then they were divided as follow:The second group was served as a positive control group. The third group was fed on a basal diet supplemented with salmon powder at the level of (5%).The fourth group was fed on a basal diet supplemented with sardine powder at the level of (5%).The fifth group was fed on a basal diet supplemented with sibia powder at the level of (5%).Results revealed that three type of fish especially sibia fish caused a significant decrease in serum urea, creatinine, uric acid, aspartate aminotransferase (AST), alanine   aminotransferase (ALT)and parathyroid hormone. In addition, a significant increase in calcium andphosphorus, vitamin D,bone mineral density and bone mineral content.In conclusion, thethree types of fish especially sibia fish protect rats against osteoporosis through its vitamin D, calcium and phosphorus content. So, it may be beneficial to the human beings.