The present study was conducted to determine the effects of calcium and vitamin D intakes on lipid profile, kidney, and liver functions in post weaning albino rats. A total of 66 male albino rats of Sprague Dawley Strain, 25 days of age and weighing (40 ± 5g) were used in this study. The rats were divided into two main groups: the first main group (n=6) fed on a basal diet (BD) and used as a control (-ve) group. The second main group (60 rats) was divided into (10 subgroups) one of them (6 rats) fed on (BD) and considered as control positive (+ve) group. The other (9 subgroups) were fed on (BD) containing different levels (50,150&200%) from calcium, vitamin D, and calcium plus vitamin D, for 8 weeks, followed by the second 8 weeks, rats were fed on high-fat diet (HFD) as the following. The positive control group (+ve) shift up to feed on (HFD), while the other (9 subgroups) were fed on (HFD) containing the same previous different levels (50,150&200%) from calcium, vitamin D, and calcium plus vitamin D.
 
At the end of the experiment, rats were sacrificed and blood samples were collected, then serum was separated, lipid profile as well as concentration of uric acid, urea nitrogen, creatinine, aspartate amino transferase (AST) and alanine amino transferase (ALT) enzymes, were determined. The obtained results revealed that feeding the early post weaning rats for the first 8 weeks on (BD) containing 50% from calcium, vitamin D & calcium plus vitamin D requirements followed by second 8 weeks fed on (HFD) containing the same low level from Ca, Vit. D & Ca plus Vit D caused a significant increase in total cholesterol (TC), Triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), very low density lipoprotein cholesterol (VLDL-c), AST and ALT, serum uric acid, urea nitrogen and creatinine, while showed a significant decrease in serum high-density lipoprotein cholesterol  (HDL-c) level. On the other hand, our results revealed a group of early post weaning rats fed for the first 8 weeks on (BD) supplemented with 150 and 200% from calcium, vitamin D& calcium plus vitamin D, followed by second 8 weeks fed on (HFD) contained the same supplementation, induced a significant decrease in (TC), (TG), LDL-c ,  VLDL-c , AST , ALT , serum uric acid , urea nitrogen      
and  creatinine , while a significant increase in the concentration of HDL-c, occurred in post weaning rats fed on (HFD) supplemented with calcium, vitamin D and calcium plus vitamin D.
 
In conclusion results taken together indicated that at early post weaning supplemented diet with calcium plus vitamin D may be have a significant effect to compate later obesity and its complications such as hyperglycemia, hypercholesterolemia and non-alcoholic fatty liver disease.