The present study aimed to evaluate the effect of propolis, curcumin and their mixtures on hepatotoxicity rats. Forty-eight adult male Sprague-Dawley rats weighing 150±10gm were divided into two main groups; the first main group (6 rats) was fed on basal diet as a negative control group. The second main group (42 rats) was fed on basal diet and injected with CCl4 (1 mL/kg b.w, 1:1 v/v mixture of CCl4 and liquid paraffin) every 72 h for 14 days to induce hepatic toxicity, then divided into 7 groups from group 2 to group 8. Group 2 was fed on basal diet as positive control group. Groups 3, 4, 5 were fed on basal diet supplemented with (1%) propolis, curcumin and their mixture from basal diet, respectively. Groups 6, 7, 8 were fed on basal diet supplemented with (2%) propolis, curcumin and their mixture from basal diet, respectively. At the end of the experimental period (4 weeks), rats were scarified and blood samples were taken for biochemical analyses. Results indicated that serum concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, total cholesterol, triglyceride, LDL-C and malondialdehyde (MDA) were significantly elevated (P<0.05) by CCl4 administration (positive control group) compared with negative control group, and significantly (P<0.05)decreased in HDL-C and glutathione peroxidase (GPX). It also indicated that supplemented diet with propolis and curcumin improved these changes that caused by CCl4 administration. We concluded that propolis and curcumin supplementation had beneficial effects on hepatotoxicity rats.