The present study aims to evaluate effect of irradiationatdoses 6 kGy and 10 kGy on chemical and biochemical parameters of sometypes ofnuts including  pistachio (Pistachiavera L.) and walnuts (JuglansregiaL.) 70albinorats used in this study, the rats divided into 7 groups as following:  Group (1): fed on basal diet, as a control group. Group (2): fed on basal diet containing un-irradiated pistachio. Groups (3 and 4): fed on diets containing the irradiated pistachio on 6 and 10kGy, respectively. Group (5): fed on basal diet containing un-irradiated walnut. Groups (6 and 7): fed on diets containing the irradiated walnut on 6 and 10 kGy, respectively for 8 weeks. The statistical analysis of irradiated pistachio and walnut at dose 6 and 10 kGy at zero time and storage for 6 months showed no significant difference in protein, moisture, ash and lipid. The biochemical performance showed non-significant effect on organs weight, total cholesterol, triacylglycerol's, high density  lipoprotein-cholesterol (HDL-c), low density lipoprotein-cholesterol (LDL-c), blood hemoglobin, glucose, serum alanine amino transferase (ALT), serum aspartate amino transferase (AST), liver superoxide dismutase (SOD), liver glutathione  peroxidase (GPX) and liver catalase in groups applied doses of non-irradiated or irradiated pistachio and walnut, as compared to control group which fed on basal diet , while serum alanine amino transferase (ALT) which showed increased in  untreatedpistachio and there were significant increase in antioxidant enzymes at all groups.