Utilization of wheat bran as by-product to produce pectin to be effective on lead acetate toxicity in rats Amira M. EJ-MosJemany Nutrition and Food Science Department, Faculty of Home Economics, AI-Azhar University, Egypt Abstract This study was carried out to be use wheat bran (rough and soft) as aby-product to produce pectin to be effective on lead acetate toxicity in rats. The biological experimental was design using male rats (36 rats) divided into six groups and it was fed on basal diet for four weeks. The first group (n= 6 rats) was administered plain water considered as negative control. The second group (n= 6 rats) was taken 0.13% lead acetate in drinking water considered as positive control. The third and fourth groups (n= 6 rats for each) was taken orally pectin 200 and 400 mg/kg/day prepared from rough wheat and it was taken 0.13% lead acetate in drinking water. The fifth and sixth groups (n= 6 rats for each) was taken orally pectin 200 and 400 mg/kg/day prepared from soft wheat) and it was taken 0.13% lead acetate in drinking water. At the end of experimental period (30 day)complete blood picture, Red blood cells (RBCs) and white blood cells (WBCs) were measured. The lipids profile, serum liver enzymes activity, kidney functions and pancreatic function were determinedand also histopathological was examined in the liver. The results showed that the lead acetate was significantly induced decreased in RBCs, WBCs counts, blood hemoglobin, hematocrit and platelets. In the