The present work was conducted to study the effect of two levels from mackerel fish, garlic and their combinations on loss of weight, serum glucose, leptin hormone, lipid fraction and kidney functions of obese rats. Forty-eight albino rats (Sprague Dawley Strain) were divided into two main groups. The first main group (6 rats) fed on basal diet was considered control negative group. The second main group (42 rats) fed on high fat diet (HFD) for 6 weeks to induce obesity. Obese rats were randomly assigned to seven equal subgroups: The first subgroup fed on HFD as positive control group, subgroups 2 and 3 fed on high fat containing 2.5% and 5% garlic powder, respectively. Subgroup 4 and 5 fed on high fat diet containing mackerel fish, which provided the diets with 50% and 100% protein, respectively. Subgroup 6 and 7fed on high fat diet containing 2.5% garlic powdered and half amount of protein from mackerel fish and 5% garlic powdered and all amount of protein from mackerel fish, respectively.Feeding rats on high fat diet led to significant increase in weights, body weight gain %, serum "glucose, leptin hormone, cholesterol, triglyceride, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, uric acid, urea nitrogen and creatinine, while high density lipoprotein cholesterol decreased, as compared to healthy rats fed on basal diet. Treating obese rats with all tested diet showed significant decrease in weight and body weight gain%, in addition to improve all parameters. The best results recorded for the group which treated with HFD containing 5% garlic powdered and 100 % of protein from mackerel fish. Our results indicated1 that garlic and mackerel fish enhances the health status of obese rats and reduced the weight gain.