The present study aimed to evaluate the effect of four concentrations of dried banana male bud (DBMB) on blood glucose level in male diabetic rats. Thirty male albino rats were divided into two main groups; first group: as control negative (n=5) and second group: diabetic rats (n=25), divided into five equal subgroups as follows: diabetic untreated rats (as control positive), diabetic rats treated with 2.5%, 5%, 7.5% and 10% DBMB. Results of the biological study revealed that injection rats with alloxan exhibited hyperglycemia and elevation in lipid profile (cholesterol, triacylglycerol & VLDL-c), liver enzymes aspartate and alanine aminotransferase (AST & ALT), and kidney function (uric acid & urea nitrogen). Treatment with the four levels of DBMB revealed gradual improvement in serum glucose, triacylglycerol, VLDL-c and all the other parameters when compared to untreated diabetic group. The most pronounced improvement was in the treated group that received the highest level of DBMB. The histopathological examination of liver, kidney and pancreas tissues confirmed this improvement at treatment with 10% DBMB. Fortified pan bread with 5% DBMB showed the best results in most of the sensory properties. In conclusion, the present study illustrates that DBMB has potent hypoglycemic activity and can be used as a supplement in the diet of diabetics.