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169604

Tenderization and fragmentation of myofibrillar proteins in camel semitendinosus muscle of high connective tissue content using proteolytic extract from ginger rhizome and papain

Article

Last updated: 23 Jan 2023

Subjects

-

Tags

Food Adulteration
Food Contaminants
Food Hygiene
Food Microbiology
Food Safety

Abstract

This study was conducted to develop a method for improving tenderness and overall qualities of tough camel's meat using plant proteolytic enzymes. 3x3 cm uniform-sized from Semitendinosus muscle of old female camel's meat "Camelus dromedaries" (over 7 years) were marinated with 7% freshly prepared ginger extract, 0.7% papain extract and mixture of papain extract (0.6%) and freshly prepared ginger extract (5%) then kept at 4°C for 3 days. The tenderized as well as the control samples were subjected to analysis in the due time in terms of sensory, bacterial, physicochemical, ultrastractural and electrophoretic examinations. Improvement of sensory scores and significant (P< 0.05) decreased APC, Coliforms, Fecal Coliforms were observed in all enzyme treated samples. Physico-chemical parameters showed significant(P< 0.05) reduction in collagen content, MFD, TBA value, shear force and significant (P< 0.05) increase total soluble protein, collagen solubility %, MFI and SL in all enzyme treated samples compared to control. Electrophoretic pattern of muscle proteins also revealed extensive proteolysis and reduction in number of protein bands in all treated samples. While, histological evaluation showed significant changes in the ultrastructure of connective tissue and muscle fiber. Mixture of both enzymes induced the most obvious effect on camel's meat tenderness followed by papain extract and finally ginger extract.

DOI

10.21608/ejfsj.2017.169604

Keywords

Tenderness/ Camel's meat/ Ginger/ Papain/ Sensory/ Bacteriology/ Physicochemical/ Ultrastructural/ Electrophoretic

Authors

First Name

Heba

Last Name

Abdel-Naeem

MiddleName

H.S.

Affiliation

Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University

Email

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City

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Orcid

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First Name

Mohamed

Last Name

Emara

MiddleName

M.T.

Affiliation

Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University

Email

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City

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Orcid

-

First Name

Adel

Last Name

Ibrahim

MiddleName

M.M.

Affiliation

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Email

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City

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Orcid

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First Name

Fathi

Last Name

El-Nawawi

MiddleName

-

Affiliation

Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University

Email

-

City

-

Orcid

-

Volume

4

Article Issue

2

Related Issue

24743

Issue Date

2017-12-01

Receive Date

2017-05-09

Publish Date

2017-12-15

Page Start

10

Page End

31

Print ISSN

2314-5676

Online ISSN

2735-5330

Link

https://ejfsj.journals.ekb.eg/article_169604.html

Detail API

https://ejfsj.journals.ekb.eg/service?article_code=169604

Order

2

Type

Original Articles

Type Code

1,676

Publication Type

Journal

Publication Title

Egyptian Journal of Food Safety

Publication Link

https://ejfsj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023