The present study aimed to investigate the efficacy of some natural essential oils on microbial vaginitis as a new promising alternative remedy. fifty cases of microbial vaginitis collected and were identified biochemically using the VITEK 2 compact system, the results showed that, 41 isolates were referred to 4 normal microflora and 37 opportunistic bacterial isolates, 7 fungal isolates and 2 parasitic infections. The antibiotics sensitivity testing was performed using different antibiotics for the pathogenic bacterial yeast isolates, The results showed that nine bacterial and two yeast isolates out of the forty-four isolates exhibited multi-drug resistance (MDR). The antimicrobial activities of the used oils against the eleven pathogenic MDR isolates, recorded that turmeric oil was the most potent essential oil againsteach microbes, which was confirmed by molecular identification. The MIC of turmeric oil against the pathogenic MDR K. Pneumoniae and C. albicans recorded that the MIC of turmeric oil was 0.781 mg/ml for both isolates. The combination between the most effective essential oils (turmeric and parsly oils) and the selected resisted antibiotics (levofloxacin and nystatin) on the most dominant MDR bacterial and yeast isolates showed increasing in the diameters of inhibition zones (from 38 to 40 mm and 10 to 24 mm) respectively. Cellular damage under SEM was conducted for the turmeric oil, which was analyzed by GC-MS to confirm its chemical composition with an abundance of Aromatic-turmerone. The present study concluded that using turmeric oil is an effective antimicrobial agent against most pathogenic MDR microbial vaginitis.