Objective: To investigate the ability of the liver extract to induce in vitro differentiation of rat adipose tissue derived mesenchymal stem cells into hepatocyte cells.
Methods: The experiment was involved 40 healthy Sprague Dawley male rats that were divided into 4 groups: group 1 was served as a normal control rats, group 2 was given intra peritoneal dose of CCl4 served as positive group, group 3 was injected with CCl4 and treated with intravenous injection of undifferentiated cells while group 4 was injected with CCl4 and differentiated AD-MSCs. Biochemical analysis was done including total bilirubin, albumin, ALT and AST. Catalase, superoxidase dismutase (SOD) and malondaialdehyde (MDA) levels were evaluated.
Results: After 21 days of in vitro differentiation of rat mesenchymal stem cells by using liver extract cells, it was observed a morphology changes: large nuclei and dark granular deposits within the cytoplasm emerged in the culture. By comparing with group 2 (+ve control); the liver function of rat group 4 was affected by injecting of differentiated MSCs by increasing albumin (p=0.001) and decreasing of ALT and AST concentrations (P = 0.0001 & 0.0001 respectively). Furthermore, there was a significant increase in the catalase and SOD levels (P=0.0001 & 0.0001 respectively), while a significant decrease in MDA was found (P=0.0001)
In conclusion: The liver extract media could differentiate AD-MSCs into hepatocytes like cells as well as biochemical activities of liver rat induced by CCl4 have an ameliorations effect by injection of in vitro differentiated MSCs.