D–π–A dye composed of pyrene and thiophene units was synthesized and characterized using elemental, FT-IR, and mass analyses. The solvatochromic response was investigated in different solvents of various polarities. Additionally, the inclusion characteristic of the investigated dye was studied in different organized assemblies of three surfactants. The spectral changes suggested that the dye would be useful to study the critical micelle concentrations of the studied assemblies systems. Furthermore, the absorption and emission spectra are sensitive towards H⁺, Co²⁺, Ni²⁺, Cd²⁺, Pb²⁺, and Zn²⁺ ions owing to acido- and metallo-chromic behaviors. These results suggest that the investigated dye would be potential candidate for polarity sensors, probe to characterize microenviromental polarity of the surfactants, and sensor for metal ions and H⁺ proton.