Scenedesmus obliquus was discussed by phycologists as a promising microalga for biodiesel
production based on its biomass and fatty acid productivity. In the present study, S. obliquus
was pilot cultivated for large scale production in a semi continuous culture for three months using
bubbled glass boxes photo- bioreactor. Cultivation of S. obliquus resulted in a maximum biomass
productivity of 0. 59 g L−1 d−1 and maximum esterified fatty acid productivity of 20.37 mg l−1 d−1
in (March, April and May), different flocculants for biomass harvest showed maximum
flocculation efficiency of 82% using 250 mg l−1 of NaOH for 2 h. Solar drying of the harvested
biomass showed significant increase of esterified fatty acid content by 50% with respect to control.
In addition, fatty acid profile and iodine number of S. obliquus oil meet biodiesel standard
specifications which make the fatty acid of S. obliquus eligible for further research to be used as a
feed stock for biodiesel production.