Aims: The primary aim of this study was to compare the effects of human platelet rich fibrin (hPRF)
exudate Vs fetal bovine serum (FBS) on the proliferation and osteodifferentiation of human dental pulp < br />stem cells (hDPSCs). The secondary one was to determine the optimum concentration of hPRF exudate
inducing hDPSCs proliferation and osteodifferentiation.
Study design: One hundred and forty ml of blood was collected from 8 healthy donors. hPRF exudate was
prepared by the direct method. hDPSCs were isolated from impacted mandibular third molars of twelve
donors by the outgrowth method. For cell viability and proliferation rate testing, 96 well plates were used
and the assay was done in duplicate and the trial repeated four times under the same conditions. Six wells
were used to contain 10% FBS, serum free media, 1%, 5%, 10% and 20% concentrations of hPRF exudates,
respectively. The proliferation assay was carried out after 1 and 3 days by MTS tetrazolium cell
proliferation assay kit and Elisa reader. The study design for osteodifferentiation protocol was exactly as the
proliferation one and instead the assay was carried out after 7 days by alizarin red with Elisa reader.
Results: Compared to 10%FBS, 10% hPRF exudate was the optimum concentration for hDPSCs
proliferation, while 1% hPRF exudate was the optimum concentration for osteodifferentiation of hDPSCs.
Conclusion: The preparation technique of hPRF exudate is simpler and cheaper than that of FBS. Avoiding
the risk of zoonosis which may be occurred with FBS, it is recommended to use 10% hPRF exudate for
proliferation and 1% for osteodifferentiation.