Background: Acute kidney injury (AKI) patients have most elevated death indices, elongated hospital admissions, and a quicker progression of chronic kidney disease (CKD). In reaction to ischemia-reperfusion AKI, urine indicators are markedly elevated in the renal proximal tubules. Aim: To evaluate new biomarkers that can predict acute renal injury following open heart surgery. Patients and Methods: The 63 patients who underwent cardiovascular surgery for the current study were splited into double collections: the AKI group, which included 21 patients who experienced acute kidney injury following surgery, and the No AKI group, which included 42 patients who did not experience acute kidney injury following surgery. All patients have undergone thorough clinical examinations and history-taking procedures. Additionally, before and postoperative measurements of serum creatinine levels, sodium, potassium, blood urea, and urine output were made. At the ICU at 0, 3, 6, 12 and 24 hours following surgery, urinary hemojuvelin (HJV) and neutrophil gelatinase-associated lipocalin (uNGAL) levels were assessed. Results: There was no statistically significant difference between postoperative hemojuvelin at 0 hr and hemojuvelin at 3,6,12, or 24 hours postoperatively, there were a statistical significance  elevation in hemojuvelin at 3,6,12, and 24 hrs postoperatively in the AKI group compared to postoperative hemojuvelin at 0 hr. Additionally, there was a statistical significance rise  in hemojuvelin at 3,6,12, and 24 hours after surgery in the AKI group compared to postoperative hemojuvelin at 0 hours, in contrast to the non-AKI group whereas there was no statistical significance differentiation between postoperative hemojuvelin at 0 hours and postoperative hemojuvelin at 3,6,12, and 24 hours after surgical operation. The ROC curve demonstrated that hemojuvelin and NGAL are effective predictors of acute kidney injury Conclusion: According to our findings, combining urine biomarkers HJV and uNGAL can increase the clinical prediction power of AKI.