Atopic dermatitis (AD), often called atopic eczema, is one of the most common inflammatory skin conditions that affects both children and adults and may result in chronic, pruritic, and recurrent dermatitis. The objectives of this research were to determine the clinical relevance of serum and faecal calprotectin in children with AD over a range of ages, to analyse the relationship between the two, and to clarify the relationship between the two and disease severity. For this case-control research, participants were chosen from the general population whereas AD patients were drawn from the outpatient clinic of the Dermatology, Venereology, and Andrology Department at Benha University Hospitals. The findings also showed that xerosis was present in every instance of atopy, as well as pit alba in 40%, k pilaris in 38%, oral in 22%, and ocular in 22%. The levels of serum and faecal calprotectin in the AD group were considerably greater than those in the control group. We discovered no significant variations in AD group serum or faecal calprotectin concentration by age, gender, or family history. There was a positive connection between serum and faecal calprotectin and AD severity, but not with age at onset, disease duration, or disease stage. Higher concentrations of serum and faecal calprotectin were thought to be an indicator of vulnerability to and severity of AD.