This fat-soluble vitamin has a critical function to perform in human health. Vitamin D deficiency continues to be a public health issue across the globe. Beyond the well-known skeletal issues, this deficit has a wide range of implications that are currently under investigation. According to their findings, a representative sample of Egypt's healthy population has their 25-hydroxyvitamin D levels checked. Methods and Subjects: This cross-sectional study recruited a total of 90 people who appeared to be in good health. Laboratory tests included serum levels of calcium (calcium), phosphorus (phosphorus), alkaline phosphatase (ALP) and parathormone hormone (PTH) levels as well as 25 hydroxyvitamin D (Vit D) (Vit.D). As a result, there were 57 women in the sample (63.3 percent ). The average age of the participants was 34.25 years plus or minus 3.5 standard deviations. This group had a mean BMI of 28.86 SD kg/cm2. The total calcium concentration was 8.86 SD (between 7.4 and 9.5 mg/dl), while the ionised calcium concentration was 1.19 SD (between 0.97 and 1.25 mmol/l). The mean level of vitamin D was 16.87 SD (between 6.7 and 37 ng/mL). Three of the three measurements were within the normal range: P was 4.04 mg/dl, PTH 46.49 micrograms per millilitre, and ALP 215.64 micrograms per millilitre. Vit. D deficiency can be predicted by looking at factors like age, physical activity, sun exposure, and dietary intake of calcium and vitamin D (P0.05). However, there was no correlation between serum Vit. D levels and body mass index (BMI). Conclusion: The healthy Egyptian adults in the study sample had a significant deficiency in vitamin D. Vit. D deficiency was linked to decreased physical activity, decreased sun exposure, and a decreased intake of vitamin D