Using antineoplastic radiation treatment, doctors were able to alter the human body's microbial ecology and manage any infectious issues that could arise. Antibiotic susceptibility testing of Klebsiella species multi-resistant isolates is prevalent and should be done in all instances of clinically important infections caused by these microbes. Klebsiella aerogenes are predicted to be increasingly implicated as causative organisms in infections. For Klebsiella species infections resistant to other antibiotics, the use of vancomycin is the most appropriate. Gram negative bacteria, particularly Klebsiella aerogenes, have lately received a lot of interest throughout the globe because of their synthesis of several bioactive compounds that may be used in the pharmaceutical business. IC60 3.3 and 1.1 g/ml. against HCT-116 and HepG-2 cell lines, respectively, against Klebsiella aerogenes with the presence of PKS4 biosynthetic gene SHM 4-1 was shown to be active against all bacteria tested. For the synthesis and purification of a single active antimicrobial molecule by high performance liquid chromatography, mannitol, ammonium sulphate, pH 5.5, 2% inoculum size, and three days of incubation at 24°C were used. Carbapenemase was found to be the source of this compound's NMR structural information.