Many people are plagued with acne vulgaris, a common skin disorder that has a negative impact on their lives. Recently, the topics of serum folic acid and uric acid (UA) have come up. It is our goal to examine the uric acid levels in acne vulgaris patients with and without a history of folic acid deficiency in healthy individuals. The following are the research topics and procedures: The patients' group contained 60 people with mild or moderate acne vulgaris, whereas the control group included twenty healthy volunteers of the same age and sex. The worldwide acne grading system was used to determine the severity of the acne (GAGS). All individuals had their serum folic acid and uric acid tested using ELISA as well as a colorimetric method. Results: There was no statistically significant difference in age, gender, or BMI between the patients and control groups (BMI). The median folic acid and UA levels were significantly different between the patient and control groups (P= 0.001, 0.009, respectively). It was shown that individuals with a favourable family history and those with scars had substantially higher median UA (P= 0.001, 0.001). There were significant connections between BMI and GAGS score (P = 0.01, 0.007). Serum UA and folic acid, according to the results of this investigation, may have a role in the inflammation of acne. The specific method by which serum UA and folic acid rise in acne sufferers is unknown at this time, and additional research is needed to corroborate this.