According to growing evidence, fungi, are a rich source of natural chemicals with a diverse variety of biological roles. As part of our effort to separate and identify physiologically active chemicals from naturally occurring sources, 45 endophytic fungi were isolated from various sources. Surface sterilization of the obtained samples and culturing of the sterilized samples on two selective media: were used to isolate endophytic fungi. First media was malt agar media (malt extract 15 g, sea salt 24.4 g, agar 15 g, distilled water up to 1 L, pH 5.2). and potato dextrose agar (prepared as per container with the addition of sea salt 24.4 g/L). Filter-sterilized nalidixic acid (50 mg/L) and chloramphenicol (200 mg/L) were added to all media. To obtain the fungal crude extracts, the isolated fungal strains were grown on potato dextrose broth media for 15 days at 28oC. The anticholinesterase activity of the obtained ethyl acetate crude extracts was tested. Seven of the identified fungus strains showed significant anticholinesterase activity. The most potent fungal strain H1 was cultivated at 25°C on potato dextrose agar (PDA), with the key morphological traits assessed using a light microscope.