Bacterial exopolysaccharides (EPSs) are biopolymers carbohydrate with high-molecular-weight which released often secreted extracellularly by bacteria. In the presented work, ten bacterial isolates were obtained from various marine samples. The bacterial isolate (BS6), was selected for its maximum productivity which reached (5.9 g/l) after being screened for exopolysaccharide (EPS) synthesis. As a result, the antioxidant activity of EPSs was determined by measuring their DPPH free radical scavenging activity. At 120 minutes, the best score was BS6 (98.40%), while the lowest score was BS1 (24.94%). On the other hand, Three EPSs, have no antioxidant action. The synthesis and production of EPS are influenced by a variety of variables. therefore, the effect of various factors on the production of EPSs by isolate (BS6) revealed that the maximum yield of EPS was (7.2 g/l) and cell dry was (3.9 g/l) when incubation temperature was 40°C for three days, RPM was 120, pH was 7, peptone was used as a nitrogen source, and sucrose was used as a carbon source