Chronic wound healing is a severe problem in veterinary practice, therefore, a new agent for wound healing is recommended. Synthetic oligodeoxynucleotides containing one or more CpG motifs (CpG-ODN) are used to stimulate the immune system and improve skin wound healing.  Ten clinically healthy animals were used to estimate and compare the rate of wound healing. The animals were arranged into two groups (five animals each). The estimation of wound healing was carried out by clinical observation of the signs of healing in 21 days, the observation was based on the presence of sepsis and unhealthy granulation tissue formation. Histopathological findings depend on the rate of new vascularization, amount of collagen bundles, epithelial thickness and cellular component. Molecular assessment based on the expression of IL10 & TGF-β. This study aimed to evaluate the role of Class C CpG oligonucleotide as new modality to overcome the delay of chronic wound healing in canine