Background: A glomerular filtration rate (eGFR) of less than 60 ml/min/1.73 mt2 indicates chronic kidney disease (CKD). Low levels of haemoglobin (Hb) cause the kidneys to produce insufficient amounts of the hormone erythropoietin, which in turn causes anaemia. Blood iron levels may be controlled by the liver-produced peptide, Hepcidin, which regulates iron absorption in the digestive tract. Serum hepcidin levels were measured in individuals with chronic renal disease to determine their iron status. Researchers at Benha University Hospital and Benha Teaching Hospital recruited 60 patients with chronic renal disease (stages I to V) and 20 healthy volunteers for the research. The connection between HB, Iron, TIBC, and transferrin was stronger in the HD group than in the Pre HD group, whereas ferritin was considerably higher in the HD group than in the Pre HD group. The HD group and the Pre HD group differed significantly. However, when looking at Po4 and PTH, HD group had a considerable advantage over Pre HD and control groups because Ca was much greater in HD than in any of the other groups. It was found that Hepcidine correlated positively with ferritin, SGPT and SGOT, ALP, UREA and creatinine as well as proteinuria, Po4 and PTH while negatively correlating with HB, iron and TIBC and Transferrin. Hepcidine was also found to be positively associated with CRP, ferritin, CRP-SGPT, and ALP-SGOT. The involvement of hepcidin in the control of dietary iron absorption and cellular iron release has been established in this study. Hemodialysis patients' erythropoiesis is regulated by hepcidin, which is predominantly found in iron reserves.