When the pampiniform and internal spermatic veins dilate, it is known as varicocele. Among sub fertile males, varicocele is the most frequent abnormality to be discovered. A 40 kDa secreted mammalian glycoprotein known as Chitinase-3-Like-1 (CHI3L1) binds chitin but lacks the enzyme capability to catalyse chitin's degradation. YKL40 is a neutrophil-produced cytokine that has been linked to a variety of inflammatory disorders. It also serves as a biomarker for endothelium disease. This study's goal is to find out whether endothelial dysfunction has a role in the development of varicocele by measuring blood levels of YKL40 in patients with varicocele. Thirty healthy men with no clinically evident varicocele were matched for age and verified by scrotal Doppler Ultrasonography with enzyme-linked immunosorbent assay to have high serum YKL40 values (ELISA). A patient's and a control's age ranged from 25 to 50. Varicocele patients had greater serum YKL40 levels than healthy controls. Endothelial dysfunction has a role in varicocele development, as shown by this finding.