Infantile haemangioma (IH) is the most common benign vascular lesion occurring in infancy and childhood. Besides typical clinical presentation, histological findings and immune-histo-chemical cellular markers may be of interest for the characterization of each stage of IH (proliferation and involution). Galectin-3 (gal-3) is a pro-angiogenic molecule that plays an important role in vascular endothelial proliferation and angiogenesis. So this work aimed to evaluate serum level of gal-3 in patients with IH to assess its possible role in the pathogenesis of tumor. To achieve this aim, 60 subjects were included in this study, divided into two main groups. Patient group included 40 cases with IH and control group included 20 age and sex matched healthy participants. Five ml of venous blood sample was taken from each participant and the assay was done by using human gal-3 ELISA kit. Galectin-3 serum level was significantly higher in patients than controls (P=0.001).Our results reported that Gal-3 may be involved the pathogenesis of IH and it can be a possible diagnostic biomarker in IH cases.