Background: Biotin is a water-solutions vitamin used for five mammalian carboxylases as an important coenzyme. It has a major role in fatty acids, amino acids and glucose metabolism. It is a popular dietary supplement for the treatment of fragile nails and hair loss. Objectives: The present research aimed at evaluating patients with or without biotin deficiency in telegon effluvium and determining after 3 months the impact of biotin replacement treatment. Methods: 130 patients with Télégon Efluvium (group A) and 120 healthy individuals (group B) served as a control group were studied. Methods: Those subjects who had been subjected to biotin treatment three months before the survey and reported additional hair loss reasons were eliminated. All patients were assessed for hair loss by clinical diagnosis before and after replacement treatment (hair pull test and Dermoscopic evaluation). The biotin level of serum was measured using commercially available immunosorbent assay kits. Results: Mean biotin serum levels were lower than controls between the two groups in a group. The impact of biotin replacement treatment has shown that better cases are 51.5%, improved cases are 34.6% and missing cases 13.8%. Patients with insufficient serum biotin levels were substantially improved, while non-improved cases with optimum blood biotin concentrations were significantly identified. Conclusion: Our results indicate that biotin addition had a substantial impact on hair loss, particularly in individuals with telegon effluvium who had deficiencies or inadequate biotin levels.